ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of lipopolysaccharides of Bacterium prodigiosum (BP-LPS) in inhibiting tumor growth and improving immunosuppression in mice.</p><p><b>METHODS</b>In mice bearing S180 tumor and a mouse model of immunosuppression induced by cyclophosphamide (CTX), the tumor growth, indexes of the immune organs and peripheral white blood cell count were measured after intraperitoneal injection of BP-LPS.</p><p><b>RESULTS</b>Injections of BP-LPS (40 U/kg) for 8 consecutive days resulted in a significant inhibition of the tumor growth in mice bearing S180 tumor (P<0.01), with a dose-dependent increase of the spleen indexes but no obvious changes in the thymus indexes. Intraperitoneal injections of BP-LPS for 7 days inhibited the reduction of peripheral white blood cells and spleen indexes in immunosuppressive mice, but did not produce any significant changes in normal mice.</p><p><b>CONCLUSION</b>BP-LPS can inhibit the tumor growth in tumor-bearing mice and enhance the immune functions of immunosuppressive mice.</p>
Subject(s)
Animals , Female , Male , Mice , Antineoplastic Agents , Pharmacology , Immunosuppressive Agents , Pharmacology , Lipopolysaccharides , Pharmacology , Polysaccharides, Bacterial , Pharmacology , Random Allocation , Serratia , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the thin layer chromatographic (TLC) fingerprint of flavonoid constituents from Polygonatum odoratum, to set up the identification protocol of the herbal and provide scientific information for its quality control.</p><p><b>METHOD</b>The ethanol extracts were separated on silica gel G precoated plate with a mixture of toluene-ethylacetate-formic acid (5:4:1) as the mobile phase. The spots were visualized with ammonia vapor, then were examined under ultraviolet light (365 nm). The plate was scanned at wavelengths of lambdaR = 500 nm, lambdaS = 280 nm.</p><p><b>RESULT</b>A fingerprint of flavonoids of P. odoratum, with 10 specific fluorescent spots while examined under ultraviolet light, was set up.</p><p><b>CONCLUSION</b>The method can be used for quality control of P. odoratum.</p>